Anticorps Polyclonal de lapin anti-HOOK3

• Phare
• Validé par KD/KO

HOOK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15457-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of rat liver using 15457-1-AP (same clone as 15457-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 15457-1-AP (same clone as 15457-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 15457-1-AP (same clone as 15457-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 15457-1-AP (same clone as 15457-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 15457-1-AP (same clone as 15457-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 15457-1-AP (same clone as 15457-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 15457-1-AP (same clone as 15457-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 15457-1-AP (same clone as 15457-1-PBS)

  IP result of anti-HOOK3 (IP:15457-1-AP, 4ug; Detection:15457-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 15457-1-AP (same clone as 15457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15457-1-AP (HOOK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 15457-1-AP (same clone as 15457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15457-1-AP (HOOK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 15457-1-AP (same clone as 15457-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using HOOK3 antibody 15457-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15457-1-PBS cible HOOK3 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HOOK3 Protéine recombinante Ag7724
• Nom complet: hook homolog 3 (Drosophila)
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 83 kDa
• Numéro d’acquisition GenBank: BC056146
• Symbole du gène: HOOK3
• Identification du gène (NCBI): 84376
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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