Anticorps Polyclonal de lapin anti-HORMAD1

HORMAD1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, CoIP, ELISA

Conjugaison

Non conjugué

N° de cat : 13917-1-AP

Synonymes

Cancer/testis antigen 46, CT46, HORMA domain containing 1, HORMAD1, Newborn ovary HORMA protein, NOHMA, RP11 363I22.1



Applications testées

Résultats positifs en WBcellules HUVEC,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

13917-1-AP cible HORMAD1 dans les applications de WB, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène HORMAD1 Protéine recombinante Ag4914
Nom complet HORMA domain containing 1
Masse moléculaire calculée 45 kDa
Poids moléculaire observé 45 kDa
Numéro d’acquisition GenBankBC047406
Symbole du gène HORMAD1
Identification du gène (NCBI) 84072
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HORMA domain-containing proteins regulate interactions between homologous chromosomes (homologs) during meiosis in a wide range of eukaryotes [PMID:21079677]. They also implicated in other processes related to crossover formation, including DSB formation, inhibition of promiscuous formation of the synaptonemal complex (SC), and the meiotic prophase checkpoint that monitors both DSB processing and SCs [PMID:19851446]. HORMAD1 first accumulates on the chromosomes during the leptotene to zygotene stages of meiotic prophase I. As germ cells progress into the pachytene stage, HORMAD1 disappears from the synapsed chromosomal regions. However, once the chromosomes desynapse during the diplotene stage, HORMAD1 again accumulates on the chromosome axis of the desynapsed homologs [PMID:19686734]. HORMAD1 exists as various isoform and the range of molecular weight of isoforms are sbout 36-45 kDa.

Protocole

Product Specific Protocols
WB protocol for HORMAD1 antibody 13917-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Sci Adv

SKP1 drives the prophase I to metaphase I transition during male meiosis.

Authors - Yongjuan Guan
human,mouseWB,IF,CoIP

Nucleic Acids Res

SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early meiotic recombination.

Authors - Yongjuan Guan
mouseIF

Nucleic Acids Res

NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells.

Authors - Lu Chen
mouseIF

Curr Biol

Prdm9 and Meiotic Cohesin Proteins Cooperatively Promote DNA Double-Strand Break Formation in Mammalian Spermatocytes.

Authors - Tanmoy Bhattacharyya
mouseWB,IF

PLoS Genet

Phosphorylation of chromosome core components may serve as axis marks for the status of chromosomal events during mammalian meiosis.

Authors - Fukuda Tomoyuki T
mouseWB,IF

PLoS Genet

Meiotic cohesins mediate initial loading of HORMAD1 to the chromosomes and coordinate SC formation during meiotic prophase.

Authors - Yasuhiro Fujiwara