Anticorps Monoclonal anti-HORMAD1

• Phare
• Validé par KD/KO

HORMAD1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D7E12

N° de cat : 67091-1-PBS

Synonymes

2D7E12, CT46, HORMA domain-containing protein 1, HORMAD 1, NOHMA

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Galerie de données de validation

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  WB analysis of human testis using 67091-1-Ig (same clone as 67091-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 67091-1-Ig (HORMAD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67091-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 67091-1-Ig (same clone as 67091-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 67091-1-Ig (HORMAD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67091-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67091-1-Ig (same clone as 67091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67091-1-Ig (HORMAD1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67091-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67091-1-Ig (same clone as 67091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67091-1-Ig (HORMAD1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67091-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67091-1-Ig (same clone as 67091-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67091-1-Ig (HORMAD1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67091-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67091-1-PBS cible HORMAD1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HORMAD1 Protéine recombinante Ag28316
• Nom complet: HORMA domain containing 1
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC047406
• Symbole du gène: HORMAD1
• Identification du gène (NCBI): 84072
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HORMA domain-containing proteins regulate interactions between homologous chromosomes (homologs) during meiosis in a wide range of eukaryotes [PMID:21079677]. They also implicated in other processes related to crossover formation, including DSB formation, inhibition of promiscuous formation of the synaptonemal complex (SC), and the meiotic prophase checkpoint that monitors both DSB processing and SCs [PMID:19851446]. HORMAD1 first accumulates on the chromosomes during the leptotene to zygotene stages of meiotic prophase I. As germ cells progress into the pachytene stage, HORMAD1 disappears from the synapsed chromosomal regions. However, once the chromosomes desynapse during the diplotene stage, HORMAD1 again accumulates on the chromosome axis of the desynapsed homologs [PMID:19686734].

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