Anticorps Recombinant de lapin anti-HORMAD1

HORMAD1 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241117E3

N° de cat : 84003-5-RR

Synonymes

241117E3, CT46, HORMA domain-containing protein 1, HORMAD 1, NOHMA

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Galerie de données de validation

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  WB analysis of human testis using 84003-5-RR

  human testis tissue were subjected to SDS PAGE followed by western blot with 84003-5-RR (HORMAD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  FC experiment of A549 using 84003-5-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug HORMAD1 Recombinant antibody (84003-5-RR, Clone:241117E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 84003-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84003-5-RR against Human HORMAD1 were performed. The affinity constant is 2.32 nM.

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain,
• Résultats positifs en FC (Intra): cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84003-5-RR cible HORMAD1 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HORMAD1 Protéine recombinante Ag4914
• Nom complet: HORMA domain containing 1
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC047406
• Symbole du gène: HORMAD1
• Identification du gène (NCBI): 84072
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HORMA domain-containing proteins regulate interactions between homologous chromosomes (homologs) during meiosis in a wide range of eukaryotes [PMID:21079677]. They also implicated in other processes related to crossover formation, including DSB formation, inhibition of promiscuous formation of the synaptonemal complex (SC), and the meiotic prophase checkpoint that monitors both DSB processing and SCs [PMID:19851446]. HORMAD1 first accumulates on the chromosomes during the leptotene to zygotene stages of meiotic prophase I. As germ cells progress into the pachytene stage, HORMAD1 disappears from the synapsed chromosomal regions. However, once the chromosomes desynapse during the diplotene stage, HORMAD1 again accumulates on the chromosome axis of the desynapsed homologs [PMID:19686734].

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