Anticorps Polyclonal de lapin anti-HOXA9

• Phare
• Validé par KD/KO

HOXA9 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, chIP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 18501-1-AP

Synonymes

HOX1G, HOX1.7, HOX1, Homeobox protein Hox-A9, Homeobox protein Hox-1G

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Galerie de données de validation

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  WB analysis using 18501-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18501-1-AP (HOXA9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 18501-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18501-1-AP (HOXA9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse appendix using 18501-1-AP

  IP result of anti-HOXA9 (IP:18501-1-AP, 3ug; Detection:18501-1-AP 1:300) with mouse appendix tissue lysate 4000ug.

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  IHC staining of mouse skin using 18501-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 18501-1-AP (HOXA9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules EC109, cellules A431, cellules HaCaT
• Résultats positifs en IP: tissu d'appendice de souris
• Résultats positifs en IHC: tissu cutané de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• ChIP: See 3 publications below

Informations sur le produit

18501-1-AP cible HOXA9 dans les applications de WB, IHC, IP, chIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HOXA9 Protéine recombinante Ag13350
• Nom complet: homeobox A9
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC010023
• Symbole du gène: HOXA9
• Identification du gène (NCBI): 3205
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HOXA9, also known as HOX1G, belongs to the Abd-B homeobox family and transiently interacts with PRMT5 in TNF-alpha stimulated endothelial cells. HOXA9 is methylated on Arg-140 by PRMT5and methylation is critical for E-selectin induction. HOXA9 as a sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. HOXA9 is required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. HOXA9 is 30 kDa, but methylation of HOXA9 is about 35-40 kDa. (PMID: 10082572)

Publications

Species	Application	Title
human	WB	Clin Epigenetics; Comprehensive comparison between azacytidine and decitabine treatment in an acute myeloid leukemia cell line; Authors - Tina Aumer
human	WB	iScience; TIFAB accelerates MLL-AF9-Induced acute myeloid leukemia through upregulation of HOXA9.; Authors - Jinming Zhao
human	WB,chIP	Cell Signal; Aberrant TRPM4 expression in MLL-rearranged acute myeloid leukemia and its blockade induces cell cycle arrest via AKT/GLI1/Cyclin D1 pathway.; Authors - Fujue Wang ; KD Validated
human	WB	Cell Biosci; Hypoxic metabolism in human hematopoietic stem cells.; Authors - Fatih Kocabas
mouse	WB	Cell Rep; YTHDF2/3 Are Required for Somatic Reprogramming through Different RNA Deadenylation Pathways.; Authors - Jiadong Liu
human	chIP	Sci Rep; Transcription Factor HOXA9 is Linked to the Calcification and Invasion of Papillary Thyroid Carcinoma.; Authors - Yilan Jin

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