Anticorps Recombinant de lapin anti-HOXB6

HOXB6 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230433F10

N° de cat : 83155-4-RR

Synonymes

Hox 2.2, Homeobox protein Hu-2, Homeobox protein Hox-B6, Homeobox protein Hox-2B, Homeobox protein Hox-2.2

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Galerie de données de validation

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  IF Staining of HepG2 using 83155-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HOXB6 antibody (83155-4-RR, Clone: 230433F10 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  IF Staining of A431 using 83155-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using HOXB6 antibody (83155-4-RR, Clone: 230433F10 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  FC experiment of K562 using 83155-4-RR

  1x10^6 K562 were intracellularly stained with 0.25 ug HOXB6 Recombinant antibody (83155-4-RR, Clone:230433F10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83155-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83155-4-RR against Human HOXB6 were performed. The affinity constant is 0.186 nM.

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2, cellules A431
• Résultats positifs en FC (Intra): K562,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83155-4-RR cible HOXB6 dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HOXB6 Protéine recombinante Ag14189
• Nom complet: homeobox B6
• Masse moléculaire calculée: 224 aa, 25 kDa
• Numéro d’acquisition GenBank: BC014651
• Symbole du gène: HOXB6
• Identification du gène (NCBI): 3216
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HOXB6 is a member of the Antp homeobox family and encodes a protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in development, including that of lung and skin, and has been localized to both the nucleus and cytoplasm. Altered expression of this gene or a change in the subcellular localization of its protein is associated with some cases of acute myeloid leukemia and colorectal cancer.

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