Anticorps Polyclonal de lapin anti-HOXB7

HOXB7 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 12613-1-AP

Synonymes

HHO.C1, Homeobox protein HHO.C1, Homeobox protein Hox-2C, Homeobox protein Hox-B7, HOX2C

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Galerie de données de validation

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  WB analysis of SKOV-3 using 12613-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 12613-1-AP (HOXB7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 12613-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 12613-1-AP (HOXB7 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IP experiment of SKOV-3 using 12613-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 12613-1-AP (HOXB7 antibody) at dilution of 1:300.

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  IF Staining of HepG2 using 12613-1-AP

  Immunofluorescent analysis of HepG2 cells using 12613-1-AP (HOXB7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules SKOV-3,
• Résultats positifs en IP: cellules SKOV-3,
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

12613-1-AP cible HOXB7 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HOXB7 Protéine recombinante Ag3245
• Nom complet: homeobox B7
• Masse moléculaire calculée: 217 aa, 24 kDa
• Poids moléculaire observé: 24-30 kDa
• Numéro d’acquisition GenBank: BC015345
• Symbole du gène: HOXB7
• Identification du gène (NCBI): 3217
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HOXB7 is a member of the homeobox family genes, which are often dysregulated in various cancer types. Particularly HOXB7 amplification and overexpression correlate with poor prognosis in various cancer such as gastric, pancreatic, and lung cancers. Moreover, HOXB7 is known to contribute to cancer progression by promoting epithelial to mesenchymal transition, anticancer drug resistance, and angiogenesis (PMID: 36659836).

Publications

Species	Application	Title
rat	WB	J Neuroinflammation; Gene expression comparison reveals distinct basal expression of HOX members and differential TNF-induced response between brain- and spinal cord-derived microvascular endothelial cells.; Authors - Yves Molino
human	WB	Int J Oncol; CircRNA hsa_circ_0070934 functions as a competitive endogenous RNA to regulate HOXB7 expression by sponging miR‑1236‑3p in cutaneous squamous cell carcinoma.; Authors - Da-Wei Zhang
human	WB	Exp Cell Res; Long non-coding RNA BBOX1-AS1 exacerbates esophageal squamous cell carcinoma development by regulating HOXB7/β-catenin axis.; Authors - Jinxiu Sheng
human	WB,IHC	Biomed Res Int; Cell Cycle-Related Gene SPC24: A Novel Potential Diagnostic and Prognostic Biomarker for Laryngeal Squamous Cell Cancer; Authors - Jialei Chen

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