Anticorps Recombinant de lapin anti-HOXB9

HOXB9 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241866C6

N° de cat : 84609-3-RR

Synonymes

HOX2E, HOX2.5, Homeobox protein Hox-B9, Homeobox protein Hox-2E, Homeobox protein Hox-2.5

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Galerie de données de validation

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  WB analysis of K-562 using 84609-3-RR

  K-562 cells were subjected to SDS PAGE followed by western blot with 84609-3-RR (HOXB9 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84609-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84609-3-RR (HOXB9 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84609-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84609-3-RR against Human HOXB9 were performed. The affinity constant is 40.5 nM.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HCT 116, cellules HT-29

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84609-3-RR cible HOXB9 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HOXB9 Protéine recombinante Ag34837
• Nom complet: homeobox B9
• Masse moléculaire calculée: 250 aa, 28 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC015565
• Symbole du gène: HOXB9
• Identification du gène (NCBI): 3219
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HOXB9 is a member of the Homeobox (HOX) gene family, which plays a crucial role in embryonic development and is involved in the regulation of gene expression during this process. In addition to its role in embryogenesis, HOXB9 has been implicated in various cancers, including breast and prostate cancer. It is overexpressed in breast cancer tissue and is regulated by estrogen, playing a critical role in angiogenesis. HOXB9 overexpression has been shown to stimulate three-dimensional colony formation in soft-agar assays, indicating its role in tumorigenesis. Furthermore, HOXB9 binds to the promoters of various tumor growth and angiogenic factors, regulating their expression, and its homeodomain plays a crucial role in transcriptional regulation and tumorigenesis.

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