Anticorps Polyclonal de lapin anti-HOXD13

HOXD13 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 18736-1-AP

Synonymes

BDE, BDSD, homeobox D13, Homeobox protein Hox 4I, Homeobox protein Hox D13, HOX4I, HOXD13, SPD

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Galerie de données de validation

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  WB analysis of A549 using 18736-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 18736-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 18736-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 18736-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 18736-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 18736-1-AP

  IP result of anti-HOXD13 (IP:18736-1-AP, 4ug; Detection:18736-1-AP 1:500) with A549 cells lysate 1600ug.

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  IHC staining of human brain using 18736-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 18736-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 18736-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 18736-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 18736-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 18736-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules Jurkat, tissu cérébral de rat, tissu cérébral de souris, tissu pulmonaire de souris
• Résultats positifs en IP: cellules A549
• Résultats positifs en IHC: tissu cérébral humain, tissu de cancer du foie humain, tissu de cirrhose hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

18736-1-AP cible HOXD13 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: homeobox D13
• Masse moléculaire calculée: 343 aa, 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC148863
• Symbole du gène: HOXD13
• Identification du gène (NCBI): 3239
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HOXD13, also named as HOX4I, belongs to the Abd-B homeobox family. HOXD13 is a sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Mutation of HOXD13 will cause synpolydactyly (SPD), brachydactyly type D (BDD), syndactyly type 5brachydactyly-syndactyly syndrome (BDSD) or brachydactyly type E (BDE).Present polyclonal anti-HOX13D antibody(18736-1-AP) is produced by immunizing animals with part of N-terminus chain of HOX13D and detect a 36-kDa band in cell and tissues.

Publications

Species	Application	Title
		Mol Pharm; Sialic Acid-Functionalized PEG-PLGA Microspheres Loading Mitochondrial Targeting Modified Curcumin for Acute Lung Injury Therapy.; Authors - Fei-Yang Jin
mouse	WB	Cell Death Dis; A novel Hoxd13 mutation causes synpolydactyly and promotes osteoclast differentiation by regulating pSmad5/p65/c-Fos/Rank axis; Authors - Lishan Zhang
human	WB	J Cancer; Knockdown of HOXD13 in Oral Squamous Cell Carcinoma Inhibited its Proliferation, Migration, and Influenced Fatty Acid Metabolism; Authors - Xingyue Ma ; KD Validated

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