Anticorps Monoclonal anti-Haptoglobin

Haptoglobin Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

3B4B9

N° de cat : 66229-1-Ig

Synonymes

BP, HAPT, haptoglobin, HP, HP2 ALPHA 2, HPA1S, Zonulin

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Galerie de données de validation

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  WB analysis of human plasma using 66229-1-Ig

  0.5 μl human plasma was subjected to SDS PAGE followed by western blot with 66229-1-Ig (HP Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66229-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66229-1-Ig (Haptoglobin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66229-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66229-1-Ig (Haptoglobin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu plasmatique humain

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66229-1-Ig cible Haptoglobin dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: singe
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Haptoglobin Protéine recombinante Ag9927
• Nom complet: haptoglobin
• Masse moléculaire calculée: 281aa,31 kDa; 228aa,25 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC058031
• Symbole du gène: HP
• Identification du gène (NCBI): 3240
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HP(Haptoglobin) is also named as zonulin and belongs to the peptidase S1 family. HP, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha(16 kDa and 9 kDa) and 2 beta(40 kDa) polypeptides that are covalently associated by disulfide bonds. In most species, apart from ruminants, Hp has a molecular mass of 100 kDa, consisting of two subunits of 40 kDa and two subunits of 9 kDa, although in a few species, such as man, genetic variant of Hp forms polymers of higher mass(PMID:2361363). Recent studies of haptoglobin show that certain oligosaccharide structures predominate in different diseases. For example, a highly-fucosylated structure is found in breast cancer and ovarian cancer, highly-sialylated structures in Crohn’s disease and highly branched structures in alcoholic liver disease and fucosylated haptoglobin is a good serum marker for pancreatic cancer.(PMID:16385567).

Publications

Species	Application	Title
monkey	WB	Int J Radiat Oncol Biol Phys; Identification of Novel Biomarkers for Acute Radiation Injury Using Multiomics Approach and Nonhuman Primate Model; Authors - Amrita K Cheema