Anticorps Polyclonal de lapin anti-HP1BP3

HP1BP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24556-1-PBS

Synonymes

Protein HP1-BP74, Protein HP1 BP74, HP1 BP74, Heterochromatin protein 1-binding protein 3

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Galerie de données de validation

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  WB analysis using 24556-1-AP (same clone as 24556-1-PBS)

  Mouse thymus lysates were subjected to SDS PAGE followed by western blot with 24556-1-AP (HP1BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24556-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 24556-1-AP (same clone as 24556-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 24556-1-AP (HP1BP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24556-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 24556-1-AP (same clone as 24556-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 24556-1-AP (HP1BP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24556-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 24556-1-AP (same clone as 24556-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24556-1-AP (HP1BP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24556-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 24556-1-AP (same clone as 24556-1-PBS)

  IP result of anti-HP1BP3 (IP:24556-1-AP, 4ug; Detection:24556-1-AP 1:300) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 24556-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 24556-1-AP (same clone as 24556-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 24556-1-AP (HP1BP3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24556-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 24556-1-AP (same clone as 24556-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HP1BP3 antibody (24556-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24556-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24556-1-PBS cible HP1BP3 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HP1BP3 Protéine recombinante Ag18709
• Nom complet: heterochromatin protein 1, binding protein 3
• Masse moléculaire calculée: 553 aa, 61 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC045660
• Symbole du gène: HP1BP3
• Identification du gène (NCBI): 50809
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Heterochromatin protein 1 binding protein 3(HP1BP3) associates with chromatin throughout the cell cycle and is required to maintain higher order chromatin structure during metaphase. HP1BP3 as a novel modulator of cognitive aging using a genetically diverse population of mice and confirm that HP1BP3 protein levels are significantly reduced in the hippocampi of cognitively impaired elderly humans relative to cognitively intact controls. Double-band staining is typical of HP1BP3 expression, indicative of multiple splice variants present in brain tissue.

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