Anticorps Polyclonal de lapin anti-VILIP3

• Phare
• Validé par KD/KO

VILIP3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 10989-1-AP

Synonymes

BDR1, Calcium binding protein BDR 1, hippocalcin like 1, Hippocalcin like protein 1, HLP2, HPCA, HPCAL1, VILIP 3, VILIP-3, Visinin like protein 3

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Galerie de données de validation

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  WB analysis using 10989-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10989-1-AP (VILIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10989-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10989-1-AP (VILIP 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10989-1-AP

  IP result of anti-VILIP3(IP:10989-1-AP, 4ug; Detection:10989-1-AP 1:10000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of human kidney using 10989-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10989-1-AP (VILIP 3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10989-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10989-1-AP (VILIP 3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse cerebellum using 10989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10989-1-AP (VILIP3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 10989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10989-1-AP (VILIP3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10989-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VILIP3 antibody (10989-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu rénal humain, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

10989-1-AP cible VILIP3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VILIP3 Protéine recombinante Ag1449
• Nom complet: hippocalcin-like 1
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC009846
• Symbole du gène: HPCAL1
• Identification du gène (NCBI): 3241
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VILIP3, is a member of neuron-specific calcium-binding proteins family found in the retina and brain. It is highly similar to human hippocalcin protein and nearly identical to the rat and mouse hippocalcin like-1 proteins. It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation and may be of relevance for neuronal signalling in the central nervous system.

Publications

Species	Application	Title
human	WB	Autophagy; Identification of HPCAL1 as a specific autophagy receptor involved in ferroptosis; Authors - Xin Chen ; KD Validated
mouse	IF	Nat Commun; Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.; Authors - I-Ling Lu
human	WB,IHC	Hepatology; Hippocalcin-like 1 suppresses hepatocellular carcinoma progression by promoting p21(Waf/Cip1) stabilization via activating ERK1/2-MAPK pathway.; Authors - Yonglong Zhang ; KD Validated
human	WB,IHC	Cancer Med; Expressional and prognostic value of HPCAL1 in cholangiocarcinoma via integrated bioinformatics analyses and experiments.; Authors - Mingjian Ma
human,mouse	WB	Theranostics; Hippocalcin-Like 1 blunts liver lipid metabolism to suppress tumorigenesis via directly targeting RUVBL1-mTOR signaling; Authors - Tao Chen
human	IHC	Signal Transduct Target Ther; Blockade of NMT1 enzymatic activity inhibits N-myristoylation of VILIP3 protein and suppresses liver cancer progression; Authors - Xiang-Peng Tan