Anticorps Monoclonal anti-HPGD

HPGD Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3F1F5

N° de cat : 66798-1-PBS

Synonymes

15 PGDH, HPGD, PGDH, PGDH1, Prostaglandin dehydrogenase 1, SDR36C1

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Galerie de données de validation

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  WB analysis using 66798-1-Ig (same clone as 66798-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66798-1-Ig (HPGD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

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  WB analysis of human small intestine using 66798-1-Ig (same clone as 66798-1-PBS)

  human small intestine tissue were subjected to SDS PAGE followed by western blot with 66798-1-Ig (HPGD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66798-1-Ig (same clone as 66798-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66798-1-Ig (HPGD antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66798-1-Ig (same clone as 66798-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66798-1-Ig (HPGD antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66798-1-Ig (same clone as 66798-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66798-1-Ig (HPGD antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66798-1-Ig (same clone as 66798-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66798-1-Ig (HPGD antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66798-1-Ig (same clone as 66798-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66798-1-Ig (HPGD antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66798-1-Ig (same clone as 66798-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66798-1-Ig (HPGD antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 66798-1-Ig (same clone as 66798-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using 66798-1-Ig (HPGD antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 66798-1-Ig (same clone as 66798-1-PBS)

  1X10^6 A549 cells were intracellularly stained with 0.2 ug Anti-Human HPGD (66798-1-Ig, Clone:3F1F5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66798-1-PBS cible HPGD dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HPGD Protéine recombinante Ag1499
• Nom complet: hydroxyprostaglandin dehydrogenase 15-(NAD)
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 25-28 kDa
• Numéro d’acquisition GenBank: BC018986
• Symbole du gène: HPGD
• Identification du gène (NCBI): 3248
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HPGD, also named as PGDH (15-hydroxyprostaglandin dehydrogenase), a prostaglandin-degrading enzyme that physiologically antagonizes COX-2 (PMID:15574495). HPGD inhibits in vivo proferation of colon cancer cells (PMID:24760190). HPGD has five isoforms with the molecular mass of 29, 21, 19, 16 and 15 kDa.

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