Anticorps Recombinant de lapin anti-HPRT

HPRT Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250656C2

N° de cat : 86049-1-PBS

Synonymes

HPRT1, EC:2.4.2.8, HGPRT, HGPRTase, Hypoxanthine-guanine phosphoribosyltransferase

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Galerie de données de validation

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  WB analysis using 86049-1-RR (same clone as 86049-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86049-1-RR (HPRT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86049-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 86049-1-RR (same clone as 86049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86049-1-RR (HPRT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86049-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 86049-1-RR (same clone as 86049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86049-1-RR (HPRT antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86049-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 86049-1-RR (same clone as 86049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86049-1-RR (HPRT antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86049-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 86049-1-RR (same clone as 86049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86049-1-RR (HPRT antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86049-1-PBS in a different storage buffer formulation.

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Informations sur le produit

86049-1-PBS cible HPRT dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HPRT Protéine recombinante Ag7044
• Nom complet: hypoxanthine phosphoribosyltransferase 1
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 24-28 kDa
• Numéro d’acquisition GenBank: BC000578
• Symbole du gène: HPRT1
• Identification du gène (NCBI): 3251
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HPRT, also named HPRT1 and HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout. Sp2/0 was reported in the literature as an HPRT1-deficient cell line. (PMID:20544509, 28045594, 27379977)

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