Anticorps Monoclonal anti-HPRT

• Phare
• Validé par KD/KO

HPRT Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C3D1

N° de cat : 67518-1-PBS

Synonymes

HPRT1, 1C3D1, EC:2.4.2.8, HGPRT, HGPRTase

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Galerie de données de validation

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  WB analysis using 67518-1-Ig (same clone as 67518-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67518-1-Ig (HPRT antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. HPRT deficient cell line Sp2/0 was served as a negative control. This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67518-1-Ig (same clone as 67518-1-PBS)

  WB result of HPRT antibody (67518-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HPRT transfected HEK-293 cells. This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 67518-1-Ig (same clone as 67518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 67518-1-Ig (same clone as 67518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 67518-1-Ig (same clone as 67518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 67518-1-Ig (same clone as 67518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67518-1-Ig (same clone as 67518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67518-1-Ig (same clone as 67518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67518-1-Ig (same clone as 67518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67518-1-Ig (same clone as 67518-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HPRT antibody (67518-1-Ig, Clone: 1C3D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67518-1-PBS cible HPRT dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HPRT Protéine recombinante Ag7044
• Nom complet: hypoxanthine phosphoribosyltransferase 1
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 24-28 kDa
• Numéro d’acquisition GenBank: BC000578
• Symbole du gène: HPRT1
• Identification du gène (NCBI): 3251
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HPRT, also named HPRT1 and HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT1 is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout. 67518-1-Ig antibody detects the protein around 28 kDa in SDS-PAGE. And HPRT1 deficient cell line Sp2/0 was served as a negative control in PTG's results.(PMID:20544509, 28045594, 27379977)

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