Anticorps Polyclonal de lapin anti-HPS6

• Phare
• Validé par KD/KO

HPS6 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 11371-1-AP

Synonymes

Hermansky Pudlak syndrome 6, HPS6, RP11 302K17.1, Ru, Ruby eye protein homolog

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Galerie de données de validation

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  WB analysis of HeLa using 11371-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11371-1-AP (HPS6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11371-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11371-1-AP (HPS6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11371-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11371-1-AP (HPS6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11371-1-AP

  IP Result of anti-HPS6 (IP:11371-1-AP, 4ug; Detection:11371-1-AP 1:300) with HeLa cells lysate 3000ug.

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  IHC staining of human ovary tumor using 11371-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11371-1-AP (HPS6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 11371-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11371-1-AP (HPS6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 11371-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11371-1-AP (HPS6 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 11371-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11371-1-AP (HPS6 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules MCF-7
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

11371-1-AP cible HPS6 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HPS6 Protéine recombinante Ag1929
• Nom complet: Hermansky-Pudlak syndrome 6
• Masse moléculaire calculée: 775 aa, 83 kDa
• Poids moléculaire observé: 83 kDa
• Numéro d’acquisition GenBank: BC011594
• Symbole du gène: HPS6
• Identification du gène (NCBI): 79803
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Hermansky-Pudlak syndrome (HPS) defines a group of at least seven autosomal recessive disorders characterized by albinism and prolonged bleeding due to defects in the lysosome-related organelles, melanosomes and platelet-dense granules, respectively. HSP6, also named as Ru, regulates the synthesis and function of lysosomes and of highly specialized organelles, such as melanosomes and platelet dense granules. It acts as cargo adapter for the dynein-dynactin motor complex to mediate the transport of lysosomes from the cell periphery to the perinuclear region.

Publications

Species	Application	Title
human	WB	Blood; vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2.; Authors - Anish Vaibhav Sharda ; KD Validated
human	WB	EMBO Rep; Rab22A recruits BLOC-1 and BLOC-2 to promote the biogenesis of recycling endosomes.; Authors - Saurabh Shakya
mouse	WB	Am J Respir Cell Mol Biol; Gene-edited MLE-15 Cells as a Model for the Hermansky Pudlak Syndromes.; Authors - Seunghyi Kook