Anticorps Monoclonal anti-HPSE

• Phare
• Validé par KD/KO

HPSE Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1D8B8

N° de cat : 66226-1-PBS

Synonymes

1D8B8, EC:3.2.1.166, Endo glucoronidase, Endo-glucoronidase, HEP

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Galerie de données de validation

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  WB analysis of HepG2 using 66226-1-Ig (same clone as 66226-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66226-1-Ig (HPSE Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66226-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66226-1-Ig (same clone as 66226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66226-1-Ig (HPSE Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66226-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66226-1-Ig (same clone as 66226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66226-1-Ig (HPSE Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66226-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66226-1-Ig (same clone as 66226-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66226-1-Ig(HPSE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66226-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66226-1-PBS cible HPSE dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HPSE Protéine recombinante Ag10067
• Nom complet: heparanase
• Masse moléculaire calculée: 543 aa, 61 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC051321
• Symbole du gène: HPSE
• Identification du gène (NCBI): 10855
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HPSE(Heparanase) is also named as HEP, HPA, HPA1, HPR1, HPSE1, HSE1 and belongs to the glycosyl hydrolase 79 family. It is a endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. HPSE is essential in the disassembly of the extracellular matrix (ECM) by invading cells. It has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 55 kDa and 53 kDa. The full length protein has six glycosylation sites. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation and glycosylation of the 50 kDa subunit appears to be essential for its solubility.

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