Anticorps Polyclonal de lapin anti-HRC

• Phare
• Validé par KD/KO

HRC Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18142-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse heart using 18142-1-AP (same clone as 18142-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 18142-1-AP (same clone as 18142-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 18142-1-AP (same clone as 18142-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 18142-1-AP (same clone as 18142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18142-1-AP (HRC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 18142-1-AP (same clone as 18142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18142-1-AP (HRC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 18142-1-AP (same clone as 18142-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 18142-1-AP (HRC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18142-1-PBS cible HRC dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HRC Protéine recombinante Ag12794
• Nom complet: histidine rich calcium binding protein
• Masse moléculaire calculée: 699 aa, 80 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC069795
• Symbole du gène: HRC
• Identification du gène (NCBI): 3270
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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