Anticorps Monoclonal anti-HRPT2, CDC73

• Phare
• Validé par KD/KO

HRPT2, CDC73 Monoclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

CloneNo.

4B4H2

N° de cat : 66490-1-PBS

Synonymes

CDC73, HRPT2, HRPT2; CDC73, 4B4H2, C1orf28

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Galerie de données de validation

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  WB analysis of HeLa using 66490-1-Ig (same clone as 66490-1-PBS)

  WB result of HRPT2, CDC73 antibody (66490-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRPT2, CDC73 transfected HeLa cells. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

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  WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

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  WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66490-1-Ig (same clone as 66490-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66490-1-Ig (same clone as 66490-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66490-1-Ig (HRPT2; CDC73 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using HRPT2, CDC73 antibody (66490-1-Ig, Clone: 4B4H2 ) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using HRPT2, CDC73 antibody (66490-1-Ig, Clone: 4B4H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66490-1-PBS cible HRPT2, CDC73 dans les applications de WB, IHC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HRPT2, CDC73 Protéine recombinante Ag2959
• Nom complet: cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• Masse moléculaire calculée: 531 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC014351
• Symbole du gène: HRPT2/CDC73
• Identification du gène (NCBI): 79577
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Parafibromin is a product of the hyperparathyroidism-jaw tumor syndrome gene HRPT2/CDC73, a putative tumor suppressor gene recently implicated in the autosomal dominant hyperparathyroidism-jaw tumor familial cancer syndrome, sporadic parathyroid cancer, and a minority of families with isolated hyperparathyroidism (PMID: 15580289). Defects in CDC73 are causes of hyperparathyroidism type 1 (HRPT1) and hyperparathyroidism type 2 (HRPT2) as well as parathyroid carcinoma (PRTC) (PMID: 12434154). Tumor suppressor parafibromin to the transcription elongation and RNA processing pathway as a PAF1 complex- and RNA polymerase II-bound protein (PMID: 15923622). Besides, parafibromin is also involved in post-transcriptional control pathways (PMID: 16116486). Recent report has revealed that pathogenic mutation, such as CDC73 gene mutation, is able to affect histone monoubiquitination (PMID: 22021426).

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