Anticorps Polyclonal de lapin anti-HSD11B1

• Phare
• Validé par KD/KO

HSD11B1 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 10928-1-AP

Synonymes

11 beta HSD1, 11 DH, HDL, HSD11, HSD11B, HSD11B1, HSD11L, SDR26C1

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Galerie de données de validation

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  WB analysis of L02 using 10928-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10928-1-AP (HSD11B1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 10928-1-AP

  WB result of HSD11B1 antibody (10928-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSD11B1 transfected L02 cells.

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  WB analysis of Jurkat using 10928-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10928-1-AP (HSD11B1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10928-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10928-1-AP (HSD11B1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 10928-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HSD11B1 antibody (10928-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules Jurkat, tissu hépatique de souris
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

10928-1-AP cible HSD11B1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSD11B1 Protéine recombinante Ag1361
• Nom complet: hydroxysteroid (11-beta) dehydrogenase 1
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC012593
• Symbole du gène: HSD11B1
• Identification du gène (NCBI): 3290
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSD11B1(Corticosteroid 11-beta-dehydrogenase isozyme 1) is also named as HSD11, HSD11L,11-DH and 11-beta-HSD1.It is a microsomal enzyme, which acts mainly as a NADP(H)- dependent reductase converting inactive cortisone (E) to active cortisol (F), thus regulating intracellular F access to glucocorticoid receptor. This enzyme is widely expressed not only in liver and adipose tissue, but also in adrenal, ovary and decidua.Western blot analysis in human liver, using a specific11-beta-HSD1 antibody, showed two bands at approximately 34 and 68 kDa confirming the dimeric nature of 11-beta-HSD1 in liver. In omental adipose tissue, an additional band of 50 kDa was strongly expressed(PMID:16914598).

Publications

Species	Application	Title
mouse	WB	Brain Behav Immun; HMGB1-mediated differential response on hippocampal neurotransmitter disorder and neuroinflammation in adolescent male and female mice following cold exposure.; Authors - Bin Xu
human	WB	J Assist Reprod Genet; Effect of luteinizing hormone concentration on transcriptome and subcellular organelle phenotype of ovarian granulosa cells.; Authors - Yu-Ting Wan
mouse	IF,WB,IHC	Int J Biol Sci; 11β-Hydroxysteroid Dehydrogenase Type 1 Facilitates Osteoporosis by Turning on Osteoclastogenesis through Hippo Signaling; Authors - Hanwen Li
human	WB	Gynecol Endocrinol; N6-methyladenosine demethylase FTO related to hyperandrogenism in PCOS via AKT pathway; Authors - Yuan-Xue Jing