Anticorps Polyclonal de lapin anti-HSD17B12

HSD17B12 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27461-1-PBS

Synonymes

17 beta HSD 12, 17-beta-HSD 12, 17-beta-hydroxysteroid dehydrogenase 12, 3 ketoacyl CoA reductase, 3-ketoacyl-CoA reductase

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Galerie de données de validation

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  WB analysis using 27461-1-AP (same clone as 27461-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27461-1-AP (HSD17B12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27461-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 27461-1-AP (same clone as 27461-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 27461-1-AP (HSD17B12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27461-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 27461-1-AP (same clone as 27461-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 27461-1-AP (HSD17B12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27461-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 27461-1-AP (same clone as 27461-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSD17B12 antibody (27461-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27461-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27461-1-PBS cible HSD17B12 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSD17B12 Protéine recombinante Ag26540
• Nom complet: hydroxysteroid (17-beta) dehydrogenase 12
• Masse moléculaire calculée: 312 aa, 34 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC012043
• Symbole du gène: HSD17B12
• Identification du gène (NCBI): 51144
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Hydroxysteroid 17-beta dehydrogenase 12 (HSD17B12, also known as KAR and SDR12C1) is a key enzyme of steroid metabolism pathway19 and is the human homolog of the yeast 3-ketoacyl-CoA reductase that catalyzes the second reaction in each VLCFA elongation cycle (PMID: 16166196; 12482854). It also acts as a drug-metabolizing enzyme (PMID: 36724833). HSD17B12 deficiency in embryonic stem cells reduced the production of arachidonic acid, a precursor of prostaglandins (PMID: 20130115).

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