Anticorps Polyclonal de lapin anti-HSD17B12

HSD17B12 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 31463-1-AP

Synonymes

EC:1.1.1.330, 3-ketoacyl-CoA reductase, 3 ketoacyl CoA reductase, 17-beta-hydroxysteroid dehydrogenase 12, 17-beta-HSD 12

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Galerie de données de validation

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  WB analysis using 31463-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31463-1-AP (HSD17B12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 31463-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31463-1-AP (HSD17B12 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 31463-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31463-1-AP (HSD17B12 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules HuH-7, cellules MDA-MB-231
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

31463-1-AP cible HSD17B12 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSD17B12 Protéine recombinante Ag34833
• Nom complet: hydroxysteroid (17-beta) dehydrogenase 12
• Masse moléculaire calculée: 312 aa, 34 kDa
• Poids moléculaire observé: ~34 kDa
• Numéro d’acquisition GenBank: BC012043
• Symbole du gène: HSD17B12
• Identification du gène (NCBI): 51144
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSD17B12 (17β-hydroxysteroid dehydrogenase 12) is an important protein belonging to the hydroxysteroid dehydrogenase family, which is mainly involved in the metabolism of steroids and fatty acids in the body. It has diverse functions in cells and is involved in various biochemical reactions, especially playing a key role in sex hormone synthesis and fatty acid elongation. HSD17B12 can convert estrone into estradiol in ovarian tissue. This enzyme is also involved in fatty acid elongation. HSD17B12 is expressed in a variety of tissues, especially in the brain tissue and the digestive system. In disease states, such as colorectal cancer (CRC), the expression level of HSD17B12 is significantly increased, suggesting that it may play a role in the occurrence and development of tumors.

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