Anticorps Polyclonal de lapin anti-HSD17B4

• Phare
• Validé par KD/KO

HSD17B4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 15116-1-AP

Synonymes

17 beta HSD 4, D bifunctional protein, DBP, EDH17B4, HSD17B4, MFE 2, MPF2, Multifunctional protein 2, SDR8C1

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Galerie de données de validation

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  WB analysis using 15116-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15116-1-AP (HSD17B4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15116-1-AP

  WB result of HSD17B4 antibody (15116-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSD17B4 transfected HepG2 cells.

• 

  WB analysis of mouse brain using 15116-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 15116-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15116-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15116-1-AP

  IP Result of anti-HSD17B4 (IP:15116-1-AP, 4ug; Detection:15116-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15116-1-AP (HSD17B4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human prostate cancer using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15116-1-AP (HSD17B4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse liver using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 15116-1-AP (HSD17B4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 15116-1-AP (HSD17B4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 15116-1-AP (HSD17B4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse heart using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 15116-1-AP (HSD17B4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15116-1-AP

  Immunofluorescent analysis of Hela cells, using HSD17B4 antibody 15116-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HepG2, tissu cardiaque de souris, tissu cérébral de souris, tissu hépatique de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu cardiaque de souris, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below

Informations sur le produit

15116-1-AP cible HSD17B4 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSD17B4 Protéine recombinante Ag7165
• Nom complet: hydroxysteroid (17-beta) dehydrogenase 4
• Masse moléculaire calculée: 80 kDa
• Poids moléculaire observé: 80 kDa, 45 kDa
• Numéro d’acquisition GenBank: BC003098
• Symbole du gène: HSD17B4
• Identification du gène (NCBI): 3295
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSD17B4 (17-beta-hydroxysteroid dehydrogenase 4) is also named as Peroxisomal multifunctional enzyme type 2, D-bifuntional protein or multifunctional protein 2. It codes for a 80 kDa enzyme containing three distinct functional domains and is localized in peroxisomes. It is a bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids and catalyzing the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-brancked-chian fatty acids. After peroxisomal import, the full-length protein is proteolytically cleaved to yield a 35-kDa dehydrogenase subunit and a 45-kDa hydratase subunit containing the hydratase and SCP domains (PMID: 28868548, 24602372).

Publications

Species	Application	Title
mouse	WB	Commun Biol; Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism; Authors - Michael Landowski
mouse	WB	Neurobiol Dis; Peroxisomal multifunctional protein-2 deficiency causes neuroinflammation and degeneration of Purkinje cells independent of very long chain fatty acid accumulation.; Authors - Verheijden Simon S
	WB	Sci Rep; Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells.; Authors - Mitsuki Nozaki
mouse	WB	Cells; Cell Type-Selective Loss of Peroxisomal β-Oxidation Impairs Bipolar Cell but Not Photoreceptor Survival in the Retina.; Authors - Daniëlle Swinkels
human	WB	Mol Cells; The Peroxisomal Localization of Hsd17b4 Is Regulated by Its Interaction with Phosphatidylserine.; Authors - Sang-Ah Lee
human	WB	Biology (Basel); In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.; Authors - Olga Gourdomichali