Anticorps Recombinant de lapin anti-HSD17B4

HSD17B4 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

251058E1

N° de cat : 86334-2-RR

Synonymes

(3R)-hydroxyacyl-CoA dehydrogenase, 17 beta HSD 4, 17-beta-HSD 4, 17-beta-hydroxysteroid dehydrogenase 4, D bifunctional protein

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Galerie de données de validation

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  WB analysis using 86334-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86334-2-RR (HSD17B4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86334-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSD17B4 antibody (86334-2-RR, Clone: 251058E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86334-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86334-2-RR against Human HSD17B4 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa, cellules HepG2, tissu hépatique de souris
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86334-2-RR cible HSD17B4 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HSD17B4 Protéine recombinante Ag7165
• Nom complet: hydroxysteroid (17-beta) dehydrogenase 4
• Masse moléculaire calculée: 80 kDa
• Poids moléculaire observé: 75 kDa, 45 kDa
• Numéro d’acquisition GenBank: BC003098
• Symbole du gène: HSD17B4
• Identification du gène (NCBI): 3295
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSD17B4 (17-beta-hydroxysteroid dehydrogenase 4) is also named as Peroxisomal multifunctional enzyme type 2, D-bifuntional protein or multifunctional protein 2. It codes for a 80 kDa enzyme containing three distinct functional domains and is localized in peroxisomes. It is a bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids and catalyzing the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-brancked-chian fatty acids. After peroxisomal import, the full-length protein is proteolytically cleaved to yield a 35-kDa dehydrogenase subunit and a 45-kDa hydratase subunit containing the hydratase and SCP domains (PMID: 28868548, 24602372).

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