Anticorps Polyclonal de lapin anti-HSD17B7

• Phare
• Validé par KD/KO

HSD17B7 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 14854-1-AP

Synonymes

17 beta HSD 7, 3 keto steroid reductase, HSD17B7, PRAP, SDR37C1

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Galerie de données de validation

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  WB analysis using 14854-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14854-1-AP (HSD17B7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14854-1-AP

  WB result of HSD17B7 antibody (14854-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSD17B7 transfected HEK-293 cells.

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  WB analysis of MCF-7 using 14854-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14854-1-AP (HSD17B7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14854-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14854-1-AP (HSD17B7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14854-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14854-1-AP (HSD17B7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 14854-1-AP

  IP Result of anti-HSD17B7 (IP:14854-1-AP, 4ug; Detection:14854-1-AP 1:1000) with MCF-7 cells lysate 3200ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat, cellules MCF-7
• Résultats positifs en IP: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

14854-1-AP cible HSD17B7 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSD17B7 Protéine recombinante Ag6639
• Nom complet: hydroxysteroid (17-beta) dehydrogenase 7
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 32-38 kDa
• Numéro d’acquisition GenBank: BC065246
• Symbole du gène: HSD17B7
• Identification du gène (NCBI): 51478
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IHC	Reprod Biol Endocrinol; The ovarian estrogen synthesis function was impaired in Y123F mouse and partly restored by exogenous FSH supplement.; Authors - Xiaoyu Tu
mouse	IF	Cell Rep; Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics.; Authors - Taha A Jan
human	WB	Nutrients; Malvidin-3-O-Glucoside Ameliorates Cadmium-Mediated Cell Dysfunction in the Estradiol Generation of Human Granulosa Cells; Authors - Shuer Liang
mouse	WB	Mol Hum Reprod; Semaphorin 4C regulates ovarian steroidogenesis through RHOA/ROCK1-mediated actin cytoskeleton rearrangement; Authors - Dan Chen