Anticorps Polyclonal de lapin anti-HSD3B1

HSD3B1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 32642-1-AP

Synonymes

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3-beta-HSD I, 3-beta-hydroxy-5-ene steroid dehydrogenase, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase

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Galerie de données de validation

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  WB analysis using 32642-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32642-1-AP (HSD3B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu placentaire humain,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

32642-1-AP cible HSD3B1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSD3B1 Protéine recombinante Ag38103
• Nom complet: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC031999
• Symbole du gène: HSD3B1
• Identification du gène (NCBI): 3283
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSD3B1, also known as hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1, is an enzyme that plays a crucial role in steroid biosynthesis. It belongs to the short-chain dehydrogenase/reductase (SDR) family and is primarily involved in the conversion of C19 and C21 steroids with a 3β-hydroxy-5-ene structure to 3-oxo-4-ene products. This enzyme is mainly localized to the placenta and non-steroidogenic tissues. It is also involved in various biological processes, including C21-steroid hormone metabolic processes, hippocampus development, and response to corticosterone. HSD3B1 has been implicated in conditions such as hypertension and hypospadias. In addition, HSD3B1 has been studied in the context of prostate cancer, where it is involved in the synthesis of androgens within the tumor, contributing to resistance to radiotherapy and other treatments. Its expression and activity can be influenced by genetic variations, which may affect clinical outcomes in patients with prostate cancer. HSD3B1 is also involved in the metabolism of oxysterols, which are oxidized forms of cholesterol or its precursors. It catalyzes the oxidation of the 3β-hydroxy group to a 3-ketone and isomerizes the double bond from Δ5 to Δ4, a key reaction in bile acid biosynthesis. This enzyme's activity is essential for the conversion of initial 3β-hydroxy stereochemistry to the 3α-hydroxy stereochemistry in primary bile acids. HSD3B1 is also involved in the metabolism of side-chain oxysterols, which are ligands to various receptors and play roles in cholesterol biosynthesis and other biological processes. Overall, HSD3B1 is a multifunctional enzyme with significant roles in steroid hormone production, oxysterol metabolism, and various disease processes.

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