Anticorps Monoclonal anti-HSD3B2

HSD3B2 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(7)

CloneNo.

2E7B4

N° de cat : 67572-1-Ig

Synonymes

2E7B4, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II, 3b HSD, 3-beta-HSD adrenal and gonadal type

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Galerie de données de validation

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  WB analysis using 67572-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 67572-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 67572-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human adrenal gland using 67572-1-Ig

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig adrenal gland using 67572-1-Ig

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 67572-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HSD3B2 antibody (67572-1-Ig, Clone: 2E7B4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, tissu de glande surrénale de porc, tissu de glande surrénale humain
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

67572-1-Ig cible HSD3B2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HSD3B2 Protéine recombinante Ag4291
• Nom complet: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC038419
• Symbole du gène: HSD3B2
• Identification du gène (NCBI): 3284
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSD3B2(3-beta-hydroxy-Delta(5)-steroid dehydrogenase) belongs to the 3-beta-HSD family and catalyzes the oxidation and isomerization of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, thus leading to the formation of all classes of steroid hormones.HSD3B2 is expressed almost exclusively in adrenals and gonads, whereas HSD3B1 is expressed predominantly in the placenta and skin(PMID:1682237). Defects in HSD3B2 are the cause of adrenal hyperplasia type 2 (AH2).

Publications

Species	Application	Title
mouse	WB	Part Fibre Toxicol; Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway.; Authors - Haibo Jin
human	WB	Ecotoxicol Environ Saf; Biochemical mechanisms of tributyltin chloride-induced cell toxicity in Sertoli cells; Authors - Pengchen Chen
human	WB	Birth Defects Res; Bisphenol A decreases progesterone synthesis in human ovarian granulosa cells.; Authors - Junfeng Qi
human	WB	Gynecol Endocrinol; N6-methyladenosine demethylase FTO related to hyperandrogenism in PCOS via AKT pathway; Authors - Yuan-Xue Jing
human	IHC	Pathol Res Pract; Disorganized adrenocortical zonational structure in COVID-19 patients: Implications of critical illness duration; Authors - Tian-Zi Wen
human	IHC,IF	Metabolism; Increased adrenal steroidogenesis and suppressed corticosteroid responsiveness in critical COVID-19; Authors - Tian-Zi Wen

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