Anticorps Monoclonal anti-HSD3B2

HSD3B2 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2E7B4

N° de cat : 67572-1-PBS

Synonymes

2E7B4, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II, 3b HSD, 3-beta-HSD adrenal and gonadal type

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Galerie de données de validation

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  WB analysis using 67572-1-Ig (same clone as 67572-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67572-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67572-1-Ig (same clone as 67572-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67572-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67572-1-Ig (same clone as 67572-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67572-1-PBS in a different storage buffer formulation.

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  WB analysis of human adrenal gland using 67572-1-Ig (same clone as 67572-1-PBS)

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67572-1-PBS in a different storage buffer formulation.

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  WB analysis of pig adrenal gland using 67572-1-Ig (same clone as 67572-1-PBS)

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67572-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 67572-1-Ig (same clone as 67572-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HSD3B2 antibody (67572-1-Ig, Clone: 2E7B4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67572-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67572-1-PBS cible HSD3B2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HSD3B2 Protéine recombinante Ag4291
• Nom complet: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC038419
• Symbole du gène: HSD3B2
• Identification du gène (NCBI): 3284
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HSD3B2(3-beta-hydroxy-Delta(5)-steroid dehydrogenase) belongs to the 3-beta-HSD family and catalyzes the oxidation and isomerization of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, thus leading to the formation of all classes of steroid hormones.HSD3B2 is expressed almost exclusively in adrenals and gonads, whereas HSD3B1 is expressed predominantly in the placenta and skin(PMID:1682237). Defects in HSD3B2 are the cause of adrenal hyperplasia type 2 (AH2).

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