Anticorps Polyclonal de lapin anti-HSF1

HSF1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 16107-1-AP

Synonymes

Heat shock factor protein 1, HSF 1, HSF1, HSTF 1, HSTF1

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Galerie de données de validation

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  WB analysis of K-562 using 16107-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 16107-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 16107-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16107-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16107-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16107-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 16107-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 16107-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 16107-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16107-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HepG2, cellules MCF-7, tissu rénal de souris, tissu splénique de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

16107-1-AP cible HSF1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSF1 Protéine recombinante Ag9023
• Nom complet: heat shock transcription factor 1
• Masse moléculaire calculée: 529 aa, 57 kDa
• Poids moléculaire observé: 68-80 kDa
• Numéro d’acquisition GenBank: BC014638
• Symbole du gène: HSF1
• Identification du gène (NCBI): 3297
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).

Publications

Species	Application	Title
human	IF	Acta Neuropathol Commun; RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases.; Authors - Mahlon Collins
human	WB	Am J Physiol Cell Physiol; Cancer associated fibroblasts suppressed ferroptosis in glioblastoma via upregulating lncRNA DLEU1; Authors - Jie Zhao
human	IHC	Ann Thorac Surg; Upregulation of Nuclear Heat Shock Factor 1 Contributes to Tumor Angiogenesis and Poor Survival in Patients With Non-Small Cell Lung Cancer.; Authors - Jingjing Cui
human	WB	Front Microbiol; The Capsid Protein VP1 of Coxsackievirus B Induces Cell Cycle Arrest by Up-Regulating Heat Shock Protein 70.; Authors - Yao Wang
rat	WB	Pharm Biol; Aspirin relieves the calcification of aortic smooth muscle cells by enhancing the heat shock response.; Authors - Quanquan Shen
human	WB, IHC	Toxicol In Vitro; Ginsenoside Rg3 induces FUT4-mediated apoptosis in H. pylori CagA-treated gastric cancer cells by regulating SP1 and HSF1 expressions.; Authors - Faisal Aziz