Anticorps Polyclonal de lapin anti-HSF1

HSF1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16107-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of K-562 using 16107-1-AP (same clone as 16107-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse spleen using 16107-1-AP (same clone as 16107-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 16107-1-AP (same clone as 16107-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 16107-1-AP (same clone as 16107-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 16107-1-AP (same clone as 16107-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 16107-1-AP (same clone as 16107-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 16107-1-AP (same clone as 16107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 16107-1-AP (same clone as 16107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 16107-1-AP (same clone as 16107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 16107-1-AP (same clone as 16107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16107-1-PBS cible HSF1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSF1 Protéine recombinante Ag9023
• Nom complet: heat shock transcription factor 1
• Masse moléculaire calculée: 529 aa, 57 kDa
• Poids moléculaire observé: 68-80 kDa
• Numéro d’acquisition GenBank: BC014638
• Symbole du gène: HSF1
• Identification du gène (NCBI): 3297
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).

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