Anticorps Monoclonal anti-HSF1

• Phare
• Validé par KD/KO

HSF1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F10A5

N° de cat : 67189-1-PBS

Synonymes

Heat shock factor protein 1, HSF 1, HSF1, HSTF 1, HSTF1

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Galerie de données de validation

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  WB analysis using 67189-1-Ig (same clone as 67189-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67189-1-Ig (HSF1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67189-1-Ig (same clone as 67189-1-PBS)

  WB result of HSF1 antibody (67189-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSF1 transfected HeLa cells. This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67189-1-Ig (same clone as 67189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67189-1-Ig (same clone as 67189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 67189-1-Ig (same clone as 67189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 67189-1-Ig (same clone as 67189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67189-1-PBS cible HSF1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HSF1 Protéine recombinante Ag29061
• Nom complet: heat shock transcription factor 1
• Masse moléculaire calculée: 529 aa, 57 kDa
• Poids moléculaire observé: 68-80 kDa
• Numéro d’acquisition GenBank: BC014638
• Symbole du gène: HSF1
• Identification du gène (NCBI): 3297
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).

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