Anticorps Polyclonal de lapin anti-HSL

HSL Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17333-1-PBS

Synonymes

LIPE, REH, LHS, Hormone-sensitive lipase, EC:3.1.1.79

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Galerie de données de validation

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  WB analysis using 17333-1-AP (same clone as 17333-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17333-1-AP (HSL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brown adipose using 17333-1-AP (same clone as 17333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 17333-1-AP (HSL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brown adipose using 17333-1-AP (same clone as 17333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 17333-1-AP (HSL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 17333-1-AP (same clone as 17333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17333-1-AP (HSL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 17333-1-AP (same clone as 17333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17333-1-AP (HSL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17333-1-PBS cible HSL dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSL Protéine recombinante Ag11360
• Nom complet: lipase, hormone-sensitive
• Masse moléculaire calculée: 117 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC070041
• Symbole du gène: HSL
• Identification du gène (NCBI): 3991
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Hormone sensitive lipase (HSL) belongs to the 'GDXG' lipolytic enzyme family. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. HSL has two isoforms with the molecular mass of 117 kDa and 84 kDa.

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