Anticorps Monoclonal anti-HSP20

HSP20 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G7D2

N° de cat : 67327-1-PBS

Synonymes

HspB6, Heat shock protein family B member 6, Heat shock protein beta-6, Heat shock protein beta 6, Heat shock 20 kDa-like protein p20

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Galerie de données de validation

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  WB analysis using 67327-1-Ig (same clone as 67327-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67327-1-Ig (HSP20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67327-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 67327-1-Ig (same clone as 67327-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67327-1-Ig (HSP20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67327-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 67327-1-Ig (same clone as 67327-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using HSP20 antibody (67327-1-Ig, Clone: 1G7D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67327-1-PBS in a different storage buffer formulation.

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  IF Staining of U-118 MG using 67327-1-Ig (same clone as 67327-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-118 MG cells using HSP20 antibody (67327-1-Ig, Clone: 1G7D2 ) at dilution of 1:1700 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67327-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50552-2

  Cytometric bead array standard curve of MP50552-2, HSPB6 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67327-1-PBS. Detection antibody: 67327-4-PBS. Standard:Ag10484. Range: 0.098-100 ng/mL.

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Informations sur le produit

67327-1-PBS cible HSP20 dans les applications de WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HSP20 Protéine recombinante Ag10484
• Nom complet: heat shock protein, alpha-crystallin-related, B6
• Masse moléculaire calculée: 160 aa, 17 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC068046
• Symbole du gène: HSP20
• Identification du gène (NCBI): 126393
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Hsp20 is a member of the small heat shock superfamily of proteins that function as chaperones to prevent protein misfolding through adenosine triphosphate−independent processes. It is widely expressed but is most abundant in cardiac, skeletal, and smooth muscles. Phosphorylation of HSP20 at Ser16 was elevated in human failing hearts as well as in murine hearts after I/R injury Indicating its cardioprotective function.

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