Anticorps Monoclonal anti-HSP47/SERPINH1

• Phare
• Validé par KD/KO

HSP47/SERPINH1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A8F6

N° de cat : 67863-1-PBS

Synonymes

HSP47, SERPINH1, 2A8F6, 47 kDa heat shock protein, Arsenic-transactivated protein 3

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Galerie de données de validation

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  WB analysis using 67863-1-Ig (same clone as 67863-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67863-1-Ig (same clone as 67863-1-PBS)

  WB result of HSP47/SERPINH1 antibody (67863-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP47/SERPINH1 transfected HeLa cells. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67863-1-Ig (same clone as 67863-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (SERPINH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67863-1-Ig (same clone as 67863-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 67863-1-Ig (same clone as 67863-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67863-1-Ig (same clone as 67863-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 67863-1-Ig (same clone as 67863-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (SERPINH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 67863-1-Ig (same clone as 67863-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 67863-1-Ig (same clone as 67863-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67863-1-Ig (same clone as 67863-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67863-1-Ig (same clone as 67863-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50781-1

  Cytometric bead array standard curve of MP50781-1, HSP47/SERPINH1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67863-2-PBS. Detection antibody: 67863-1-PBS. Standard:Ag30999. Range: 0.781-100 ng/mL.

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Informations sur le produit

67863-1-PBS cible HSP47/SERPINH1 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HSP47/SERPINH1 Protéine recombinante Ag30999
• Nom complet: serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC014623
• Symbole du gène: HSP47/SERPINH1
• Identification du gène (NCBI): 871
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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