Anticorps Monoclonal anti-HSP90

HSP90 Monoclonal Antibody for WB, IHC, IF/ICC, IP, CoIP, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(103)

CloneNo.

3F11C1

N° de cat : 60318-1-Ig

Synonymes

HSP90AA1, 3F11C1, EC:3.6.4.10, Heat shock protein family C member 1, Heat shock protein HSP 90 alpha



Applications testées

Résultats positifs en WBcellules LNCaP, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, cellules ROS1728
Résultats positifs en IPcellules HepG2,
Résultats positifs en IHCtissu de tumeur ovarienne humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HepG2,
Résultats positifs en CoIPtissu cérébral de souris

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
COImmunoprécipitation (IP)COIP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

60318-1-Ig cible HSP90 dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

Réactivité Humain, porc, rat, souris
Réactivité citéerat, Humain, porc, poulet, singe, souris
Hôte / Isotype Mouse / IgG2a
Clonalité Monoclonal
Type Anticorps
Immunogène HSP90 Protéine recombinante Ag3826
Nom complet heat shock protein 90kDa alpha (cytosolic), class A member 1
Masse moléculaire calculée 853 aa, 90 kDa
Poids moléculaire observé 85-90 kDa
Numéro d’acquisition GenBankBC023006
Symbole du gène HSP90
Identification du gène (NCBI) 3320
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application. This antibody may cross-react with HSP90AB1 due to the high homology between them.

Protocole

Product Specific Protocols
WB protocol for HSP90 antibody 60318-1-IgDownload protocol
IHC protocol for HSP90 antibody 60318-1-IgDownload protocol
IF protocol for HSP90 antibody 60318-1-IgDownload protocol
IP protocol for HSP90 antibody 60318-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia

Authors - Tiemei Li
mouse,humanWB

Sci Adv

YAP drives fate conversion and chemoresistance of small cell lung cancer.

Authors - Qingzhe Wu
ratIF

Dev Cell

Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination

Authors - Li Li
humanWB

Aging Cell

Urolithin A improves mitochondrial health, reduces cartilage degeneration, and alleviates pain in osteoarthritis.

Authors - Davide D'Amico
humanWB,RIP,IP

Mol Cancer

Exosomal circSHKBP1 promotes gastric cancer progression via regulating the miR-582-3p/HUR/VEGF axis and suppressing HSP90 degradation.

Authors - Mengyan Xie
mouse,humanWB,IF

Redox Biol

Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.

Authors - Zhongren Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Xiao (Verified Customer) (06-03-2024)

Works well in many cell types

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HT1080 and HEK293T
FH

Boyan (Verified Customer) (10-05-2021)

This antibody recognized a strong band around 90 kd, as shown in the website.

  • Applications: Western Blot
FH

Ayyappan (Verified Customer) (01-16-2019)

This antibody works well for IP in human cancer cell lines

  • Applications: Immunoprecipitation,
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