Anticorps Monoclonal anti-HSP90

HSP90 Monoclonal Antibody for WB, IHC, IF/ICC, IP, CoIP, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

CloneNo.

3F11C1

N° de cat : 60318-1-PBS

Synonymes

HSP90AA1, 3F11C1, EC:3.6.4.10, Heat shock protein family C member 1, Heat shock protein HSP 90 alpha

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Galerie de données de validation

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  WB analysis using 60318-1-Ig (same clone as 60318-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60318-1-Ig (HSP90 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 60318-1-Ig (same clone as 60318-1-PBS)

  IP result of anti-HSP90 (IP:60318-1-Ig, 5ug; Detection:60318-1-Ig 1:5000) with HepG2 cells lysate 1200 ug. This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 60318-1-Ig (same clone as 60318-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60318-1-Ig (HSP90 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 60318-1-Ig (same clone as 60318-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60318-1-Ig (HSP90 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60318-1-Ig (same clone as 60318-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60318-1-Ig (HSP90 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

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  CoIP experiment of mouse brain using 60318-1-Ig (same clone as 60318-1-PBS)

  Co-IP result of anti-HSP90 (IP:60318-1-Ig, 4ug; Detection:13422-1-AP 1:5000) with mouse brain tissue lysate 2240ug. This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60318-1-PBS cible HSP90 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HSP90 Protéine recombinante Ag3826
• Nom complet: heat shock protein 90kDa alpha (cytosolic), class A member 1
• Masse moléculaire calculée: 853 aa, 90 kDa
• Poids moléculaire observé: 85-90 kDa
• Numéro d’acquisition GenBank: BC023006
• Symbole du gène: HSP90
• Identification du gène (NCBI): 3320
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application. This antibody may cross-react with HSP90AB1 due to the high homology between them.

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