Anticorps Monoclonal anti-HSP90

• Phare
• Validé par KD/KO

HSP90 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

2D3D1

N° de cat : 67450-1-Ig

Synonymes

HSP90AB1, 2D3D1, Heat shock protein family C member 3, Heat shock protein HSP 90 beta, Heat shock protein HSP 90-beta

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Galerie de données de validation

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  WB analysis using 67450-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67450-1-Ig (HSP90AB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67450-1-Ig

  WB result of HSP90AB1 antibody (67450-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP90AB1 transfected HEK-293 cells.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules Neuro-2a, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

67450-1-Ig cible HSP90 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HSP90 Protéine recombinante Ag28379
• Nom complet: heat shock protein 90kDa alpha (cytosolic), class B member 1
• Masse moléculaire calculée: 723 aa, 83 kDa
• Poids moléculaire observé: 83-90 kDa
• Numéro d’acquisition GenBank: BC014485
• Symbole du gène: HSP90AB1
• Identification du gène (NCBI): 3326
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSP90, encoded by HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.

Publications

Species	Application	Title
human	WB	Virol Sin; Hsp90 β is Critical for the Infection of Severe Fever with Thrombocytopenia Syndrome Virus; Authors - Bo Wang ; KD Validated

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