Anticorps Recombinant de lapin anti-HSP90

• Phare
• Validé par KD/KO

HSP90 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, poisson-zèbre, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5P14

N° de cat : 80301-1-PBS

Synonymes

HSP90AB1, 5P14, Heat shock protein family C member 3, Heat shock protein HSP 90 beta, Heat shock protein HSP 90-beta

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Galerie de données de validation

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  WB analysis of zebrafish using 80301-1-RR (same clone as 80301-1-PBS)

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 80301-1-RR (HSP90AB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

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  WB analysis using 80301-1-RR (same clone as 80301-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80301-1-RR (HSP90AB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 80301-1-RR (same clone as 80301-1-PBS)

  WB result of HSP90AB1 antibody (80301-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP90AB1 transfected HEK-293 cells. This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 80301-1-RR (same clone as 80301-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80301-1-RR (HSP90AB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80301-1-RR (same clone as 80301-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HSP90AB1 antibody (80301-1-RR, Clone: 5P14 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 80301-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80301-1-RR against Human HSP90AB1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

80301-1-PBS cible HSP90 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, poisson-zèbre, rat, souris

• Réactivité: Humain, poisson-zèbre, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HSP90 Protéine recombinante Ag1947
• Nom complet: heat shock protein 90kDa alpha (cytosolic), class B member 1
• Masse moléculaire calculée: 723 aa, 83 kDa
• Poids moléculaire observé: 83-90 kDa
• Numéro d’acquisition GenBank: BC014485
• Symbole du gène: HSP90AB1
• Identification du gène (NCBI): 3326
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HSP90, encoded by HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.

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