Anticorps Polyclonal de lapin anti-GRP94

• Phare
• Validé par KD/KO

GRP94 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF-P, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 10979-1-AP

Synonymes

HSP90B1, 94 kDa glucose-regulated protein, ECGP, Endoplasmin, GP96

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Galerie de données de validation

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  WB analysis using 10979-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10979-1-AP (GRP94 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10979-1-AP

  WB result of GPR94 antibody (10979-1-AP, 1:1000) with si-control and si-GPR94 transfected HEK293 cells..

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  WB analysis of MCF-7 using 10979-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10979-1-AP (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10979-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10979-1-AP (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10979-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10979-1-AP (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10979-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10979-1-AP (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 10979-1-AP

  IP result of anti-GRP94 (IP:10979-1-AP, 4ug; Detection:10979-1-AP 1:500) with MCF-7 cells lysate 4000ug.

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  IHC staining of human breast cancer using 10979-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10979-1-AP (HSP90B1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 10979-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10979-1-AP (GRP94 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10979-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10979-1-AP (GRP94 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10979-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10979-1-AP (HSP90B1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of human appendicitis using 10979-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using GRP94 antibody (10979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse pancreas using 10979-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using GRP94 antibody (10979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U2OS using 10979-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using GRP94 antibody (10979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  FC experiment of MCF-7 using 10979-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug GRP94 Polyclonal antibody (10979-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules K-562, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de côlon humain, tissu cérébral de souris, tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu pancréatique de souris, tissu d'appendicite humain
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

10979-1-AP cible GRP94 dans les applications de WB, IHC, IF-P, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GRP94 Protéine recombinante Ag1439
• Nom complet: heat shock protein 90kDa beta (Grp94), member 1
• Masse moléculaire calculée: 96 kDa
• Poids moléculaire observé: 94 kDa
• Numéro d’acquisition GenBank: BC009195
• Symbole du gène: GRP94
• Identification du gène (NCBI): 7184
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glucose-regulated protein 94 (GRP94), also called HSP90B1 and GP96, is an endoplasmic reticulum (ER)-resident member of the heat shock protein 90 (HSP90) family (PMID: 33802964; 24658275). Under ER stress conditions, GRP94 accelerates its function as a molecular chaperone. Client proteins of GRP94 include toll-like receptors (TLRs), glycoprotein (GP) IX subunit, insulin-like growth factors (IGFs), proinsulin, and integrins (PMID: 7913987; 32781621; 22079671). As well as being an ER chaperone, GRP94 can also participate in Calcium regulation and is also a regulator of innate and adaptive immunity (PMID: 33802964; 11584270).

Publications

Species	Application	Title
mouse	WB	J Neuroinflammation; TRPV4 contributes to ER stress and inflammation: implications for Parkinson's disease.; Authors - Na Liu
pig,human	WB	Vet Microbiol; ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells.; Authors - Liang Zhang
rat	WB	Mol Med Rep; Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats.; Authors - Maojun Liu
mouse	WB,IHC,IF	J Immunol Res; Negative Feedback of the cAMP/PKA Pathway Regulates the Effects of Endoplasmic Reticulum Stress-Induced NLRP3 Inflammasome Activation on Type II Alveolar Epithelial Cell Pyroptosis as a Novel Mechanism of BLM-Induced Pulmonary Fibrosis; Authors - Qiaohui Hong
human	WB	Carcinogenesis; Extracellular Vesicles Long RNA Profiling Identifies Abundant mRNA, circRNA and lncRNA in Human Bile as Potential Biomarkers for Cancer Diagnosis; Authors - Huajie Zong
human	WB	Exp Brain Res; CircNCOA4 knockdown attenuates OGD-induced neuron injury through miR-338-5p/PDE4B axis; Authors - Danyang Wang

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