Anticorps Monoclonal anti-GRP94

GRP94 Monoclonal Antibody for FC, IF, IHC, WB,ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

1H10B7

N° de cat : 60012-1-Ig

Synonymes

ECGP, GP96, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1

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Galerie de données de validation

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  WB analysis of human heart using 60012-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 60012-1-Ig (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 60012-1-Ig

  human liver tissue were subjected to SDS PAGE followed by western blot with 60012-1-Ig (HSP90B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 60012-1-Ig

  human lung tissue were subjected to SDS PAGE followed by western blot with 60012-1-Ig (HSP90B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 60012-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 60012-1-Ig (GRP94 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 60012-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60012-1-Ig (GRP94 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 60012-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60012-1-Ig (GRP94 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 60012-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60012-1-Ig (GRP94 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 60012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60012-1-Ig (GRP94 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 60012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60012-1-Ig (GRP94 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60012-1-Ig (GRP94 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60012-1-Ig (GRP94 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 60012-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60012-1-Ig (GRP94 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 60012-1-Ig

  Immunofluorescent analysis of HepG2 cells using 60012-1-Ig (GRP94 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

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  IF Staining of HeLa using 60012-1-Ig

  Immunofluorescent analysis of Hela cells using 60012-1-Ig (GRP94 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

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  IF Staining of MCF-7 using 60012-1-Ig

  Immunofluorescent analysis of MCF-7 cells using 60012-1-Ig (GRP94 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

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  FC experiment of HeLa using 60012-1-Ig

  1X10^6 HeLa cells were stained with 0.2ug GRP94 antibody (60012-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: Coeur humain, cellules NIH/3T3, cellules RAW 264.7, cellules ROS1728, tissu cardiaque humain, tissu hépatique humain, tissu pulmonaire humain
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules HeLa, cellules MCF-7
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

60012-1-Ig cible GRP94 dans les applications de WB, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GRP94 Protéine recombinante Ag1439
• Nom complet: heat shock protein 90kDa beta (Grp94), member 1
• Masse moléculaire calculée: 96 kDa
• Poids moléculaire observé: 95 kDa
• Numéro d’acquisition GenBank: BC009195
• Symbole du gène: HSP90B1
• Identification du gène (NCBI): 7184
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Précipitation de l'acide caprylique/du sulfate d'ammonium
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.

Publications

Species	Application	Title
human	WB	Autophagy; Influenza M2 protein regulates MAVS-mediated signaling pathway through interacting with MAVS and increasing ROS production.; Authors - Ruifang Wang
human	WB	Front Cell Dev Biol; Extracellular Vesicles Carry lncRNA SNHG16 to Promote Metastasis of Breast Cancer Cells via the miR-892b/PPAPDC1A Axis.; Authors - Wenfei Xia
rat	CoIP	Neurochem Res; Novel Role of HAX-1 in Neurons Protection After Spinal Cord Injury Involvement of IRE-1.; Authors - Jiajia Chen
human	WB	J Adv Res; Development of a novel thymidylate synthase (TS) inhibitor capable of up-regulating P53 expression and inhibiting angiogenesis in NSCLC.; Authors - Xin-Yang Li
mouse	WB	Cell Rep; Hepatic DNAJB9 Drives Anabolic Biasing to Reduce Steatosis and Obesity.; Authors - Fangfang Sun
		Front Physiol; Evidence for Sexual Dimorphism in the Response to TLR3 Activation in the Developing Neonatal Mouse Brain: A Pilot Study.; Authors - Raul Chavez-Valdez