Anticorps Polyclonal de lapin anti-HSPA13

• Phare
• Validé par KD/KO

HSPA13 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 12667-2-AP

Synonymes

Heat shock 70 kDa protein 13, Heat shock protein family A member 13, Microsomal stress-70 protein ATPase core, STCH, Stress-70 protein chaperone microsome-associated 60 kDa protein

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Galerie de données de validation

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  WB analysis using 12667-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12667-2-AP (HSPA13 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12667-2-AP

  IP result of anti-HSPA13 (IP:12667-2-AP, 4ug; Detection:12667-2-AP 1:300) with HeLa cells lysate 1200ug.

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  IHC staining of human endometrial cancer using 12667-2-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12667-2-AP (HSPA13 antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules K-562
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de l'endomètre humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

12667-2-AP cible HSPA13 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSPA13 Protéine recombinante Ag3349
• Nom complet: heat shock protein 70kDa family, member 13
• Masse moléculaire calculée: 471 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC036370
• Symbole du gène: HSPA13
• Identification du gène (NCBI): 6782
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSPA13 (Heat shock 70 kDa protein 13), also known as STCH (the stress 70 protein chaperone), is a member of the Hsp70 family of ATPase "stress" or "heat shock" proteins. Human STCH encodes a 60 kDa protein that is highly enriched in the ER. Recently it has been reported that overexpression of the HSPA13 gene reduces prion disease incubation time in mice.

Publications

Species	Application	Title
human	WB	Sci Adv; HSPA13 facilitates NF-κB-mediated transcription and attenuates cell death responses in TNFα signaling.; Authors - Chun Gao ; KO Validated
human	WB	Proc Natl Acad Sci U S A; Overexpression of the Hspa13 (Stch) gene reduces prion disease incubation time in mice.; Authors - Grizenkova Julia J
human	WB	Endocrinology; Reassessment of the Proteomic Composition and Function of Extracellular Vesicles in the Seminal Plasma.; Authors - Hanshu Wang
human	WB,IHC	Proteome Sci; PRM-based quantitative proteomics analysis of altered HSP abundance in villi and decidua of patients with early missed abortion; Authors - Xiao-Fang Chen
human	WB,IHC,CoIP	Cell Death Discov; Heat shock protein HSPA13 promotes hepatocellular carcinoma progression by stabilizing TANK; Authors - Xuesong Cen ; KD Validated
human	WB	Endocrinology; Human seminal extracellular vesicles enhance endometrial receptivity through leukemia inhibitory factor; Authors - Hanshu Wang

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