Anticorps Recombinant de lapin anti-HSPA1B

HSPA1B Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242371E8

N° de cat : 84873-4-RR

Synonymes

242371E8, HSP72

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Galerie de données de validation

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  WB analysis using 84873-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84873-4-RR (HSPA1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84873-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84873-4-RR against Human HSPA1B were performed. The affinity constant is 9.62 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HEK-293, cellules HepG2, cellules K-562, tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84873-4-RR cible HSPA1B dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HSPA1B Protéine recombinante Ag13700
• Nom complet: heat shock 70kDa protein 1B
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC057397
• Symbole du gène: HSP70
• Identification du gène (NCBI): 3304
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSP70 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSP70 is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSP70 protein can be highly activated by various stressful stimuli. Significant up-regulation of HSP70 has been found in various tumors. Recently it has been reported that HSP70 can be constitutively expressed in selected cell types. HSP70 is also used as exosomal marker.

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