Anticorps Monoclonal anti-HSPA1L

HSPA1L Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

3E7H2

N° de cat : 66780-1-Ig

Synonymes

3E7H2, Heat shock 70 kDa protein 1-Hom, Heat shock 70 kDa protein 1-like, Heat shock protein family A member 1L, HSP70 1L

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Galerie de données de validation

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  WB analysis of HeLa using 66780-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66780-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 66780-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66780-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66780-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66780-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 66780-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66780-1-Ig (HSPA1L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 66780-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66780-1-Ig (HSPA1L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66780-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSPA1L antibody (66780-1-Ig, Clone: 3E7H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules L02, cellules NIH/3T3
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

66780-1-Ig cible HSPA1L dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HSPA1L Protéine recombinante Ag5182
• Nom complet: heat shock 70kDa protein 1-like
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC034483
• Symbole du gène: HSPA1L
• Identification du gène (NCBI): 3305
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSPA1L (Hsp70-like protein 1, also known as HSP70-hom) is a member of Hsp70 family of heat shock proteins. Recently it has been reported that HSPA1L can interact with TLR4, activate dendritic cells and induce Th1 Response. This antibody well recognizes the endogenous HSPA1L protein in multiple cell lines. (21730052)

Publications

Species	Application	Title
mouse	IF	J Cachexia Sarcopenia Muscle; The regulation of MFG-E8 on the mitophagy in diabetic sarcopenia via the HSPA1L-Parkin pathway and the effect of D-pinitol; Authors - Wenqian Zhao

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