Anticorps Polyclonal de lapin anti-HSPA2

• Phare
• Validé par KD/KO

HSPA2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 12797-1-AP

Synonymes

Heat shock 70 kDa protein 2, heat shock 70kDa protein 2, HSP70 2, HSP70 3, HSPA2

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Galerie de données de validation

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  WB analysis of HeLa using 12797-1-AP

  WB result of HSPA2 antibody (12797-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPA2 transfected HeLa cells.

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  WB analysis using 12797-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12797-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12797-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 12797-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 12797-1-AP

  IP Result of anti-HSPA2 (IP:12797-1-AP, 4ug; Detection:12797-1-AP 1:1000) with mouse skeletal muscle tissue lysate 4000ug.

• 

  IHC staining of human testis using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate hyperplasia using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse ovary using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 12797-1-AP

  1X10^6 HeLa cells were stained with 0.2ug HSPA2 antibody (12797-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules C6, cellules HeLa, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, tissu cérébral humain, tissu de muscle squelettique humain, tissu rénal humain
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu de tumeur ovarienne humain, tissu d'hyperplasie de la prostate humain, tissu ovarien de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 6 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

12797-1-AP cible HSPA2 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSPA2 Protéine recombinante Ag3539
• Nom complet: heat shock 70kDa protein 2
• Masse moléculaire calculée: 639 aa, 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC036107
• Symbole du gène: HSPA2
• Identification du gène (NCBI): 3306
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)

Publications

Species	Application	Title
mouse	IF	Sci Adv; Circular RNAs from BOULE play conserved roles in protection against stress-induced fertility decline.; Authors - Liuze Gao
human	IHC	Cell Death Differ; RNF144A functions as a tumor suppressor in breast cancer through ubiquitin ligase activity-dependent regulation of stability and oncogenic functions of HSPA2.; Authors - Yin-Long Yang ; KD Validated
human	WB	Curr Biol; RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.; Authors - Long Jiang
		J Mol Cell Biol; Cigarette smoke-induced cell cycle arrest in spermatocytes [GC-2spd(ts)] is mediated through cross talk between Ahr-Nrf2 pathway and MAPK signaling.; Authors - Prabagaran Esakky
mouse	WB,IHC	Development; Mutation of Eif4g3, encoding a eukaryotic translation initiation factor, causes male infertility and meiotic arrest of mouse spermatocytes.; Authors - Sun Fengyun F
human	WB, IHC	Int J Mol Sci; Various Anti-HSPA2 Antibodies Yield Different Results in Studies on Cancer-Related Functions of Heat Shock Protein A2.; Authors - Dorota Scieglinska ; KD Validated