Anticorps Monoclonal anti-HSPA2

HSPA2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A4A9

N° de cat : 66291-1-PBS

Synonymes

Heat shock 70 kDa protein 2, heat shock 70kDa protein 2, HSP70 2, HSP70 3, HSPA2

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Galerie de données de validation

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  WB analysis using 66291-1-Ig (same clone as 66291-1-PBS)

  Various lysates (25 μg/lane) were subjected to SDS PAGE followed by western blot with 66291-1-Ig (HSPA2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 66291-1-Ig (same clone as 66291-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66291-1-Ig (HSPA2 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66291-1-Ig (same clone as 66291-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66291-1-Ig (HSPA2 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66291-1-Ig (same clone as 66291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66291-1-Ig (HSPA2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66291-1-Ig (same clone as 66291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66291-1-Ig (HSPA2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66291-1-Ig (same clone as 66291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66291-1-Ig (HSPA2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66291-1-Ig (same clone as 66291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66291-1-Ig (HSPA2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66291-1-PBS cible HSPA2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HSPA2 Protéine recombinante Ag24228
• Nom complet: heat shock 70kDa protein 2
• Masse moléculaire calculée: 639 aa, 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC036107
• Symbole du gène: HSPA2
• Identification du gène (NCBI): 3306
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)

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