Anticorps Recombinant de lapin anti-HSPA2

• Phare
• Validé par KD/KO

HSPA2 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

240421A2

N° de cat : 83466-1-RR

Synonymes

HSP70 2, Heat shock-related 70 kDa protein 2, Heat shock protein family A member 2, Heat shock 70 kDa protein 2, 240421A2

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Galerie de données de validation

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  WB analysis of HeLa using 83466-1-RR

  WB result of HSPA2 antibody (83466-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPA2 transfected HeLa cells.

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  WB analysis using 83466-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83466-1-RR (HSPA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 83466-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83466-1-RR (HSPA2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 83466-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83466-1-RR (HSPA2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83466-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83466-1-RR against Human HSPA2 were performed. The affinity constant is 1.86 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules A549, cellules HaCaT, cellules NIH/3T3, tissu rénal humain
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83466-1-RR cible HSPA2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HSPA2 Protéine recombinante Ag3539
• Nom complet: heat shock 70kDa protein 2
• Masse moléculaire calculée: 639 aa, 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC036107
• Symbole du gène: HSPA2
• Identification du gène (NCBI): 3306
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. At physiological temperature, the HSPA2 was localized primarily in cytoplasm whereas, during heat shock, localization shifted to nucleus and nucleoli (PMID: 18452162). HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)

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