Anticorps Monoclonal anti-HSPA4

HSPA4 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain, porc

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2F2C12

N° de cat : 67173-1-Ig

Synonymes

APG 2, APG2, Heat shock 70 kDa protein 4, heat shock 70kDa protein 4, HS24/P52, HSP70, hsp70RY, HSPA4, RY

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Galerie de données de validation

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  WB analysis using 67173-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67173-1-Ig (HSPA4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67173-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67173-1-Ig (HSPA4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 67173-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67173-1-Ig (HSPA4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules Jurkat, cellules L02, tissu cérébral de porc

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67173-1-Ig cible HSPA4 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HSPA4 Protéine recombinante Ag16624
• Nom complet: heat shock 70kDa protein 4
• Masse moléculaire calculée: 840 aa, 94 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC110861
• Symbole du gène: HSPA4
• Identification du gène (NCBI): 3308
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Chromatographie d'affinité thiophilique
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSPA4 (also known as Apg-2, HSP70RY) is a 110 kDa cytosolic protein, a member of the heat-shock protein 110 (Hsp 110) subfamily of Hsp70 proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA4 is ubiquitously expressed and its expression is not heat inducible. Overexpression of HSPA4 has been reported in some leukemia and solid tumors.