Anticorps Polyclonal de lapin anti-HSPA4L

HSPA4L Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 32468-1-AP

Synonymes

APG1, Heat shock 70 kDa protein 4L, Heat shock 70-related protein APG-1, Heat shock protein family H member 3, Heat-shock protein family A member 4-like protein

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Galerie de données de validation

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  WB analysis using 32468-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32468-1-AP (HSPA4L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 32468-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 32468-1-AP (HSPA4L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 32468-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 32468-1-AP (HSPA4L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 32468-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 32468-1-AP (HSPA4L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules K-562, cellules U-251
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

32468-1-AP cible HSPA4L dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSPA4L Protéine recombinante Ag38354
• Nom complet: heat shock 70kDa protein 4-like
• Masse moléculaire calculée: 95kDa，839aa
• Poids moléculaire observé: 95~100 kDa
• Numéro d’acquisition GenBank: NM_001317381.1
• Symbole du gène: HSPA4L
• Identification du gène (NCBI): 22824
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSPA4L (Heat shock 70-kDa protein 4-like), also known as APG1 or OSP94, is a member of the heat shock protein 110 family, whose mRNA is strongly expressed in normal human testis and overexpressed in leukemia cells. HSPA4L was also restrictedly expressed in normal tissues and the its product was capable of eliciting a humoral immune response likely induced by its high-level expression in leukemia cells. Up-regulation of the Hspa4l gene in kidney and renal cell lines by osmotic stress suggested that Hspa4l may function to enable the kidney to compensate for osmotic stress present within the kidney (PMID: 16923965, 17588478).

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