Anticorps Monoclonal anti-HSPA6

HSPA6 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

3D12D7

N° de cat : 68257-1-Ig

Synonymes

Heat shock 70 kDa protein 6, Heat shock 70 kDa protein B, HSP70B, HSPA6

---

Galerie de données de validation

• 

  WB analysis using 68257-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68257-1-Ig (HSPA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 68257-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68257-1-Ig (HSPA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 68257-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 68257-1-Ig (HSPA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 68257-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using HSPA6 antibody (68257-1-Ig, Clone: 3D12D7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293, cellules HeLa, cellules HepG2, cellules MCF-7
• Résultats positifs en IF: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

68257-1-Ig cible HSPA6 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HSPA6 Protéine recombinante Ag4716
• Nom complet: heat shock 70kDa protein 6 (HSP70B')
• Masse moléculaire calculée: 643 aa, 71 kDa
• Poids moléculaire observé: 68-71 kDa
• Numéro d’acquisition GenBank: BC035665
• Symbole du gène: HSPA6
• Identification du gène (NCBI): 3310
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSPA6 (also known as HSP70B) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Human HSP70B is known to be strictly inducible and shows very low or no basal expression levels in most cells. HSPA6 induction has been exploited as a reasonable biomarker of cellular stresses and its mRNA expression levels have been shown to be significantly induced against a variety of cellular stresses. This antibody was raised against the C-terminal region of human HSPA6 and it may cross-react with HSPA1 (HSP70), HSPA1L and HSPA2. (18229458)

Publications

Species	Application	Title
human	WB,IHC	Biochim Biophys Acta Mol Cell Res; PP4R1 accelerates the malignant progression of NSCLC via up-regulating HSPA6 expression and HSPA6-mediated ER stress; Authors - Xunxia Zhu