Anticorps Polyclonal de lapin anti-Hsc70

• Phare
• Validé par KD/KO

Hsc70 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

58

Avis (2)

N° de cat : 10654-1-AP

Synonymes

Heat shock 70 kDa protein 8, heat shock 70kDa protein 8, Heat shock cognate protein 71 kDa, HSC54, HSC70, HSC71, HSP71, HSP73, HSP7C, HSPA10, HSPA8, LAP1

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Galerie de données de validation

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  WB analysis using 10654-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10654-1-AP (Hsc70 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10654-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10654-1-AP (Hsc70 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10654-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10654-1-AP (Hsc70 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10654-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10654-1-AP (Hsc70 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10654-1-AP

  IP result of anti-Hsc70(IP:10654-1-AP, 4ug; Detection:10654-1-AP 1:2000) with HEK-293 cells lysate 1640 ug.

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  IHC staining of human renal cell carcinoma using 10654-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10654-1-AP (Hsc70 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 10654-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10654-1-AP (Hsc70 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10654-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10654-1-AP (Hsc70 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10654-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10654-1-AP (Hsc70 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 10654-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using Hsc70 antibody (10654-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules MCF-7, cellules MG U-87, cellules NIH/3T3, cellules PC-12, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de carcinome à cellules rénales humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 46 publications below
• IHC: See 5 publications below
• IF: See 11 publications below
• IP: See 4 publications below
• CoIP: See 5 publications below

Informations sur le produit

10654-1-AP cible Hsc70 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Hsc70 Protéine recombinante Ag1018
• Nom complet: heat shock 70kDa protein 8
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC007276
• Symbole du gène: HSPA8
• Identification du gène (NCBI): 3312
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson's disease, indicating its implication in neurodegenerative disease (21832061). In addition, this antibody is most likely able to recognize Hsp70.

Publications

Species	Application	Title
human	WB	Nature; A dominant autoinflammatory disease caused by non-cleavable variants of RIPK1.; Authors - Panfeng Tao
		J Extracell Vesicles; Human bronchial epithelial cell-derived extracellular vesicle therapy for pulmonary fibrosis via inhibition of TGF-β-WNT crosstalk.; Authors - Tsukasa Kadota
mouse	WB	Cell Rep Med; Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing; Authors - Dongming Lv
human	WB	Genes Dev; Chaperone-mediated autophagy degrades mutant p53.; Authors - Vakifahmetoglu-Norberg Helin H
mouse	WB	Redox Biol; WBP2 restrains the lysosomal degradation of GPX4 to inhibit ferroptosis in cisplatin-induced acute kidney injury; Authors - Zebin Deng
human	WB,IF	Redox Biol; Glia maturation factor-β induces ferroptosis by impairing chaperone-mediated autophagic degradation of ACSL4 in early diabetic retinopathy.; Authors - Caiying Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

I had a very strong and specific Hsc70 band at ~70 kDa. Definitely one of the best antibodies I have used for Hsp70 family proteins.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Heart

Hsc70 Antibody Western Blot validation (1:1000 dilution) in Heart (Cat no:10654-1-AP)

FH

Mayur (Verified Customer) (04-30-2019)

Great antibody. Detects the HSC70 expression in the H4 cells very clearly.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H4

Hsc70 Antibody Western Blot, validation (1:1000 dilution) in H4 (Cat no:10654-1-AP)

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